Time-dependent regulation of perineuronal nets in the cerebellar cortex during abstinence of cocaine-self administration

The probability of structural remodeling in brain circuits may be modulated by molecules perineuronal nets (PNNs) that restrict neuronal plasticity to stabilize circuits. Animal research demonstrates addictive drugs can remodel PNNs different regions, including the cerebellum. This study aimed investigate effects short versus extended access cocaine self-administration on PNN expression around Golgi interneurons cerebellar cortex after periods abstinence. After 1 week training (2 h/day), Sprague-Dawley rats self-administered daily for 20 days under (ShA) or (LgA) access. cerebellum was assessed day, 7 days, and 28 forced were immunolabeled using Wisteria floribunda agglutinin (WFA) captured confocal microscopy. WFA intensity increased PNN-bearing neurons over abstinence period a higher proportion more intense formed throughout first month 24 h abstinence, however, we found reduction with ShA as compared naïve animals. When comparing rats, LgA showed consistent upregulation at Our results suggest produces modifications enhance conditions synaptic cortex. These are revealed shortly cessation drug intake but become during protracted group, pointing stabilization drug-induced changes. findings indicate dynamically regulates